Role of aflR Gene Expression from A. flavus to Cause Disease in Human
ثبت نشده
چکیده
1Key Laboratory of Pathogenic Fungi and Mycotoxins of Fujian Province, Fujian Agriculture and Forestry University, China 2Key Laboratory of Biopesticide and Chemical Biology of Education Ministry, and School of Life Science, Fujian Agriculture and Forestry University, China 3Key Laboratory of Agroecological Processing and Safety Monitoring, Fujian Agriculture and Forestry University, China 4Institute of Applied Ecology and Research centre of Biodiversity and Eco-Safety, Fujian Agriculture and Forestry University, China
منابع مشابه
Expression of aflR, veA and laeA as regulators of aflatoxins and cyclopiazonic acid biosynthesis pathway in Aspergillus flavus
In this study, the production of aflatoxin B1 (AFB1) and cyclopiazonic acid (CPA) was investigated in toxigenic and non-toxigenic Aspergillus flavus with respect to expression of aflR, veA and laeA genes that are involved to toxins production. A. flavus strains were cultured in YES broth at 28 °C for 4 days and the presence of (AFB1) and (CPA) was confirmed and measured by TLC and HPLC. The exp...
متن کاملInhibitory Effect of Vitamin C on Aspergillus parasiticus Growth and Aflatoxin Gene Expression
Objective: Aflatoxin is known as one of the most important mycotoxins that threatens of human life. The toxin is produced by Aspergillus species which are common cause of contamination of agricultural products. For this reason, the use of organic compounds has always been considered in order to inhibit the growth of fungi and production of toxin. The aim of this study was to investigate the eff...
متن کاملSilencing of the aflatoxin gene cluster in a diploid strain of Aspergillus flavus is suppressed by ectopic aflR expression.
Aflatoxins are toxic secondary metabolites produced by a 70-kb cluster of genes in Aspergillus flavus. The cluster genes are coordinately regulated and reside as a single copy within the genome. Diploids between a wild-type strain and a mutant (649) lacking the aflatoxin gene cluster fail to produce aflatoxin or transcripts of the aflatoxin pathway genes. This dominant phenotype is rescued in d...
متن کاملApplication of PCR on detection of aflatoxinogenic fungi
Aflatoxins are carcinogenic metabolites produced by several strains of Aspergillus flavus group in food and feed. Cluster genes in aflatoxin biosynthesis pathway contain structural, regular and unassigned genes , nor-1 , ver-1 , and omt-1 are structural genes that coding for key enzymes and aflR is a regulatory gene that plays a key role in the production of aflatoxin and is affecting on the s...
متن کاملExpression of genes of the aflatoxin biosynthetic pathway in Aspergillus flavus isolates from keratitis
PURPOSE To document transcriptional activation (expression) of key aflatoxin biosynthetic pathway genes in corneal isolates of Aspergillus flavus. METHODS The expression of certain regulatory (aflatoxin regulatory [aflR] and aflatoxin J [aflJ]) and structural (polyketide synthase acetate [pksA] and norsolonic acid-1 [nor-1]) genes in four corneal A. flavus isolates was evaluated by reverse tr...
متن کامل